Journal: Antimicrobial Agents and Chemotherapy

Article Title: Spheroplast-Mediated Carbapenem Tolerance in Gram-Negative Pathogens

doi: 10.1128/AAC.00756-19

Figure Lengend Snippet: Bacterial isolates evaluated in this study a

Article Snippet: Our results suggest that measurement of antibiotic susceptibility and, ultimately, treatment outcome could include more-nuanced responses, such as tolerance, in Gram-negative pathogens. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Isolate Carbapenemase Specimen source MHA MIC value (μg/ml) MHA MIC value interpretation e BHI + agar MIC value (μg/ml) BHI + agar MIC value interpretation f E. cloacae complex ARB0008 b NA NA 1 SUS 1.5 NA E. cloacae complex ATCC 13047 NA NA 0.064 SUS 0.094 NA E. cloacae complex WCM0001 NA Blood 0.023 SUS 0.023 NA E. coli WCM0001 NA Urine 0.016 SUS 0.016 NA E. coli TUV93-0 NA NA 0.023 SUS 0.023 NA K. aerogenes ARB0007 NA NA 0.064 SUS 0.047 NA K. aerogenes WCM0001 NA Respiratory/sinus 0.032 SUS 0.032 NA K. pneumoniae WCM0001 NA Blood 0.047 SUS 0.032 NA K. pneumoniae WCM0002 NA Respiratory/sinus 0.032 SUS 0.064 NA P. aeruginosa PA14 NA NA 0.25 SUS 0.25 NA V. cholerae N16961 NA NA 0.125 SUS 0.125 NA E. cloacae complex 41952 KPC NA >32 RES >32 NA E. coli 52862 KPC NA >32 RES 32 NA K. aerogenes 28944 KPC NA >32 RES >32 NA K. pneumoniae ARB0120 c KPC NA 16 RES 16 NA P. aeruginosa ARB0090 d KPC NA >32 RES >32 NA Open in a separate window a ARB, Centers for Disease Control and Prevention and U.S. Food and Drug Administration Antibiotic Resistance Isolate Bank; ATCC, American Type Culture Collection; BHI + , brain heart infusion agar with supplements; MHA, Mueller-Hinton agar; NA, not applicable; RES, resistant; SUS, susceptible; WCM, Weill Cornell Medicine. b For ARB0008, the meropenem MIC value determined by the ARB is 2 μg/ml (intermediate).

Techniques:

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Spheroplast-Mediated Carbapenem Tolerance in Gram-Negative Pathogens

doi: 10.1128/AAC.00756-19

Figure Lengend Snippet: Meropenem-induced spheroplasts can recover to form an exponentially growing population. A time-lapse montage of spheroplasts imaged upon removal of meropenem after 6 h of treatment is shown. The antibiotic was removed by addition of purified NDM-1 carbapenemase, followed by time-lapse microscopy on BHI+ agarose pads (0.8% [wt/vol] agarose). Images were then acquired 5 min apart for another 2 h. Both E. coli isolates were omitted since no spheroplasts were observed after 6 h of meropenem treatment. Scale bars, 5 μm.

Article Snippet: Our results suggest that measurement of antibiotic susceptibility and, ultimately, treatment outcome could include more-nuanced responses, such as tolerance, in Gram-negative pathogens. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Isolate Carbapenemase Specimen source MHA MIC value (μg/ml) MHA MIC value interpretation e BHI + agar MIC value (μg/ml) BHI + agar MIC value interpretation f E. cloacae complex ARB0008 b NA NA 1 SUS 1.5 NA E. cloacae complex ATCC 13047 NA NA 0.064 SUS 0.094 NA E. cloacae complex WCM0001 NA Blood 0.023 SUS 0.023 NA E. coli WCM0001 NA Urine 0.016 SUS 0.016 NA E. coli TUV93-0 NA NA 0.023 SUS 0.023 NA K. aerogenes ARB0007 NA NA 0.064 SUS 0.047 NA K. aerogenes WCM0001 NA Respiratory/sinus 0.032 SUS 0.032 NA K. pneumoniae WCM0001 NA Blood 0.047 SUS 0.032 NA K. pneumoniae WCM0002 NA Respiratory/sinus 0.032 SUS 0.064 NA P. aeruginosa PA14 NA NA 0.25 SUS 0.25 NA V. cholerae N16961 NA NA 0.125 SUS 0.125 NA E. cloacae complex 41952 KPC NA >32 RES >32 NA E. coli 52862 KPC NA >32 RES 32 NA K. aerogenes 28944 KPC NA >32 RES >32 NA K. pneumoniae ARB0120 c KPC NA 16 RES 16 NA P. aeruginosa ARB0090 d KPC NA >32 RES >32 NA Open in a separate window a ARB, Centers for Disease Control and Prevention and U.S. Food and Drug Administration Antibiotic Resistance Isolate Bank; ATCC, American Type Culture Collection; BHI + , brain heart infusion agar with supplements; MHA, Mueller-Hinton agar; NA, not applicable; RES, resistant; SUS, susceptible; WCM, Weill Cornell Medicine. b For ARB0008, the meropenem MIC value determined by the ARB is 2 μg/ml (intermediate).

Techniques: Purification, Time-lapse Microscopy

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Spheroplast-Mediated Carbapenem Tolerance in Gram-Negative Pathogens

doi: 10.1128/AAC.00756-19

Figure Lengend Snippet: Spheroplast formation and recovery in human serum. (A and B) The indicated isolates were grown overnight in serum growth medium (SGM), diluted 1:10 (final volume, 500 μl) into fresh SGM, and incubated in the presence (A) or absence (B) of 10 μg/ml meropenem. Cells were plated (CFU per milliliter) at the indicated time points. (C) After 6 h of incubation, purified NDM-1 carbapenemase was added to remove meropenem, followed by time-lapse microscopy on agarose pads containing 40% (vol/vol) human serum. All values represent means of results from three biological replicates; error bars represent standard errors of the means. The red circle indicates the initial growth steps of a recovering spheroplast within cell debris.

Article Snippet: Our results suggest that measurement of antibiotic susceptibility and, ultimately, treatment outcome could include more-nuanced responses, such as tolerance, in Gram-negative pathogens. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Isolate Carbapenemase Specimen source MHA MIC value (μg/ml) MHA MIC value interpretation e BHI + agar MIC value (μg/ml) BHI + agar MIC value interpretation f E. cloacae complex ARB0008 b NA NA 1 SUS 1.5 NA E. cloacae complex ATCC 13047 NA NA 0.064 SUS 0.094 NA E. cloacae complex WCM0001 NA Blood 0.023 SUS 0.023 NA E. coli WCM0001 NA Urine 0.016 SUS 0.016 NA E. coli TUV93-0 NA NA 0.023 SUS 0.023 NA K. aerogenes ARB0007 NA NA 0.064 SUS 0.047 NA K. aerogenes WCM0001 NA Respiratory/sinus 0.032 SUS 0.032 NA K. pneumoniae WCM0001 NA Blood 0.047 SUS 0.032 NA K. pneumoniae WCM0002 NA Respiratory/sinus 0.032 SUS 0.064 NA P. aeruginosa PA14 NA NA 0.25 SUS 0.25 NA V. cholerae N16961 NA NA 0.125 SUS 0.125 NA E. cloacae complex 41952 KPC NA >32 RES >32 NA E. coli 52862 KPC NA >32 RES 32 NA K. aerogenes 28944 KPC NA >32 RES >32 NA K. pneumoniae ARB0120 c KPC NA 16 RES 16 NA P. aeruginosa ARB0090 d KPC NA >32 RES >32 NA Open in a separate window a ARB, Centers for Disease Control and Prevention and U.S. Food and Drug Administration Antibiotic Resistance Isolate Bank; ATCC, American Type Culture Collection; BHI + , brain heart infusion agar with supplements; MHA, Mueller-Hinton agar; NA, not applicable; RES, resistant; SUS, susceptible; WCM, Weill Cornell Medicine. b For ARB0008, the meropenem MIC value determined by the ARB is 2 μg/ml (intermediate).

Techniques: Incubation, Purification, Time-lapse Microscopy

Journal: BMC Microbiology

Article Title: The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

doi: 10.1186/1471-2180-13-164

Figure Lengend Snippet: DNA:DNA relatedness percentages between representatives of two novel Enterobacter species and closely-related species

Article Snippet: For cellular fatty acid analysis, the six novel strains, next to three type strains from species of the genus Enterobacter (i.e. E. cloacae subsp. cloacae ATCC 13047 T , E. radicincitans D5/23 T and E. arachidis Ah-143 T ) were cultivated in triplicate on plates containing TSB (trypticase soy broth) amended with 15 g of agar (TSBA) at 30°C for about 24 h. Fatty acid methyl esters (FAME) from strains at the same physiological stage were extracted and prepared by the instant FAME TM protocol of the Microbial Identification System (MSI, Microbial ID, Inc., Newark, Delaware, USA; http://www.midi-inc.com/pages/mis_literature.html ).

Techniques:

Journal: BMC Microbiology

Article Title: The new species Enterobacter oryziphilus sp. nov. and Enterobacter oryzendophyticus sp. nov. are key inhabitants of the endosphere of rice

doi: 10.1186/1471-2180-13-164

Figure Lengend Snippet: Key reactions for biochemical differentiation of selected Enterobacter species

Article Snippet: For cellular fatty acid analysis, the six novel strains, next to three type strains from species of the genus Enterobacter (i.e. E. cloacae subsp. cloacae ATCC 13047 T , E. radicincitans D5/23 T and E. arachidis Ah-143 T ) were cultivated in triplicate on plates containing TSB (trypticase soy broth) amended with 15 g of agar (TSBA) at 30°C for about 24 h. Fatty acid methyl esters (FAME) from strains at the same physiological stage were extracted and prepared by the instant FAME TM protocol of the Microbial Identification System (MSI, Microbial ID, Inc., Newark, Delaware, USA; http://www.midi-inc.com/pages/mis_literature.html ).

Techniques: